Antibacterial activity of crude extracts of euphorbia hirta against. Isolation of phenolic compounds of patikan kebo leaves. Its chemistry, traditional and medicinal uses, and pharmacological activities. Bioassayguided fractionation and isolation of antibacterial. Among the three solvent extracts, methanol extract of euphorbia hirta showed 1015 mm inhibition against b.
This study was carried out to investigate the antibacterial activities of both ethanol and water extracts of euphorbia hirta against some pathogens. Antibacterial activity of crude extracts of euphorbia. Phytochemical screening and antimicrobial activity of. Biologically important plants were discovered by evaluation. Euphorbia hirta has been widely used by tribal as traditional medicine in a treatment against infectious pathogens. Leaf, flower, stem and root extracts for their antibacterial and antifungal activity and brine shrimp lethality mohammad abu basma rajeh 1, zakaria zuraini 1, sreenivasan sasidharan 2, lachimanan yoga latha 2 and santhanam amutha 2,3. The presence of antibacterial substances in the higher plants is well established 17. All the tested extracts showed varying degrees of strain. This work was therefore planned to produce a protocol for callus induction from leaf of e. Antibacterial assay was carried out via agar well diffusion assay for screening purpose and finally through micro dilution method in order to determine minimum. Minimum inhibitory concentration mic of extracts was by broth microdilution method. Flavonoid extracts from euphorbia hirta were screened for antibacterial activity by disc. As far as our literature survey could ascertain, the antimicrobial and cytotoxic activity of e.
Antibacterial activity, euphorbia hirta, crude extracts, bacterial isolates. The ethanol extract of the leaves of euphorbia hirta was studied for its antimicrobial activity by agar well diffusion method against. Phytochemical study and screening for antimicrobial activity of. More work needs to be done on the separation and purification of active compounds in the plant extract in order to determine the role of each compound in the crude extracts. Euphorbia hirta, phytochemical, methanolic extract, hexane extract, aqueous extract, enteric.
Flavonoid extracts from euphorbia hirta were screened for antibacterial activity by disc diffusion assay. Staphylococcus aureus mtcc 2940, bacillus ceresus, salmonella typhi mtcc 733, klebsiella pneumoniae mtcc9. Phytochemical and antibacterial studies of different parts. Pharmacology and therapeutic potential of euphorbia hirta. Antibacterial, antioxidant activity and phytochemical analysis of euphorbia hirta linn savita jakhar 1 and pushpa dahiya 1. The water extracts provided the higher yield and also more antibacterial effectiveness than when organic solvents were used. The antibacterial activity was detected with spread plate well diffusion method. Antimicrobial activity and cytotoxicity evaluation of euphorbia hirta. The ethanolic extract exhibited the strongest antimicrobial activity against salmonella typhi with mic value of 0. Ethanomedicinal plant against gram negative uti pathogens article pdf available january 2014 with 370 reads how we measure reads. Extracts found sensitive against test pathogens were also evaluated for minimum bactericidal concentration mbc and total activity ta. Euphorbia hirta powdered plant material was extracted using 3 solvents methanol, hexane and distilled water.
Keywords euphorbia hirta, antibacterial, antifungal, drug resistance abstract the antibacterial and antifungal effect of euphorbia hirta was evaluated against a panel of bacteria escherichia coli, psueudomonas aeruginosa, klebsiella pneumoniae and staphylococcus aureus and fungi aspergillus avus, fusarium semitectum and trichophyton. Antibacterial activity the antimicrobial activities of e. Two species of plants, andrographis paniculata and euphorbia hirta were screened for antibacterial activities against three gram positives and gram negatives. Antibacterial, antioxidant activity and phytochemical. Euphorbia maculata, variously called spotted spurge or prostrate spurge, the latter name not to be confused with euphorbia prostrata, is a fastgrowing annual. Euphorbia hirta, phytochemical, methanolic extract, hexane extract, aqueous extract, enteric infections, antimicrobial activity. Keywords euphorbia hirta, antibacterial, antifungal, drug resistance abstract the antibacterial and antifungal effect of euphorbia hirta was evaluated against a panel of bacteria escherichia coli, psueudomonas aeruginosa, klebsiella pneumoniae and staphylococcus aureus and fungi aspergillus avus, fusarium semitectum and trichophyton sp. Antibacterial activity of euphorbia hirta eprints usm. Herbal medicine is the oldest form of medicine known to mankind. Significantly little or no zone of inhibition was observed when e.
Phytochemical screening of the crude extracts revealed the presence of tannins, saponins, phenolics, flavonoids, cardiac glycosides. The leaves of euphorbia hirta and erigeron floribundus were collected during the late rainy season in 1995 in ekpoma, and identified in the botany. In vitro antimicrobial activity and phytochemical analysis. In contrast, hexane extract appeared to be the least active extract as its inhibition potency was been observed only on proteus vulgaris. Antibacterial activities and toxicological potentials of.
It was the mainstay of many early civilizations and still the most widely practiced form of medicine in the world today. Antibacterial assay was carried out via agar well diffusion assay for screening purpose and finally through. The leaves from both plants were extracted by methanol extraction. Phytochemical studies showed the presence of cardiac glycosides, steroidsphytosterols, anthocyanin, terpenoids, flavonoids and tannins. Antibacterial activity of euphorbia fusiformisa rare. Euphorbia hirta plant extracts using three solvents methanol, hexane and. Invitro antimicrobial activities of euphorbia hirta. Antimicrobial and phytochemical investigation of the. Antibacterial activity of andrographis paniculata and. There are several published reports describing the antimicrobial activity of various crude plant extracts 2,3.
This study aims to phytochemical and antimicrobial study of euphorbia hirta euphorbiaceae. Leaf, flower, stem and root extracts for their antibacterial and antifungal activity and brine shrimp lethality. P jayraman, director of plant anatomy research centre, and chennai. The agar diffusion method was used to determine the antibacterial activity against staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, salmonella typhi and bacillus subtilis at different concentrations while toxicological studies was carried out through intraperitoneal injection of albino rat with varying concentrations of the extract. Euphorbiaceae and isolation of its bioactive constituents by shanmugapriya perumal thesis. Antimicrobial activity of leaf extracts of euphorbia hirta l. The present study was aimed at evaluating the antimicrobial and cytotoxic activities of various extracts of euphorbia hirta.
The present study is carried out by evaluation of euphorbia hirta and calotropisprocera against mdr cultures. Pdf antibacterial activity of euphorbia hirta extracts. Evaluation of antibacterial properties of euphorbia condylocarpa. Molecules free fulltext assessment of euphorbia hirta. The antimicrobial activities of the methanolic extracts of euphorbia hirta l leaves, flowers, stems and roots were evaluated against some medically important bacteria and yeast using the agar disc diffusion method. Evaluation of antimicrobial activity of euphorbia hirta and calotropis procera against mdr pathogens 1amit pandey and2nehaverma mrd life sciences pvt ltd, lucknow, 226010 abstract. The agar diffusion method was used to determine the antibacterial activity against staphylococcus aureus, escherichia coli. Antibacterial activity of polygonum plebejum and euphorbia hirta against staphylococcus aureus mrsa article pdf available in journal of pure and applied microbiology 124.
Antibacterial activity of polygonum plebejum and euphorbia. Mic of plant extract polygonum plebeium pp and euphorbia hirta eh was 25mg ml. Materials and methods preparation of plant extracts euphorbia hirta leaves were used for the present study. Phytochemical study and screening for antimicrobial. Microbiology research journal international, 24567043,vol 20, issue 2. The aim of the current study was to investigate antibacterial activity of the ethanol and petroleum ether extracts of euphorbia hirta l against clinically important bacterial sp. Leaves of euphorbia hirta used in traditional medicine for the treatments of boils, wounds and control of diarrhoea and dysentery were extracted by maceration in ethanol. Four gram positive staphylococcus aureus, micrococcus sp. Antimicrobial activity and cytotoxicity evaluation of. An ornamental plant euphorbia milii euphorbiaceae is plays a role in folk medicine. Antibacterial activities and phytochemical screening of. Antibacterial and antifungal activity of euphorbia hirta l.
Euphorbia hirta, antibacterial, disc diffusion, minimum inhibitory concentration. Pdf antibacterial activity of euphorbia hirta extracts researchgate. The alcoholic extract of euphorbia hirta leaves is highly effective against gram positive bacteria and moderately effective against gram negative bacteria. Ethnobotanical information of some traditionally used indian medicinal plant species selected for antibacterial activity. The study reveals the profound antimicrobial activity of aqueousethanol extract of ten different plant species. Pdf deadly diseases of this century were mainly caused by bacterial and fungal infections. Antibacterial sensitivity test indicated that the extracts of e. The chinese use it as a cure for cancer, and some brazilians believe that it can cure warts. Antibacterial on euphorbia hirta is one of plants which is abundant in the tropical area. There are several reports stating that other euphorbia species extracts exhibit antibacterial activity. It is a potent medicinal plant and has established its sedative and anxiolytic activity, analgesic, antipyretic, antiinflammatory, antidepressant for blood pressure. Leaves of euphorbia hirta used in traditional medicine for the treatment of boils, wounds and control of diarrhoea and dysentery was extracted by maceration in ethanol. Bismi sonia nazeer guest lecturer, department of botany, bishop moore college, mavelikkara. The healthy, fresh leaves of selected medicinal plants were collected, dried under shade and dried leaves were homogenized to a fine powder.
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